Hair testing is the way to go for testing PEDs'

[mikemurni]

you really are a ******, PEDs are different they need to find ways to detect them in the samples they take. All we know is you can detect some PEDs in hair, not which ones and not the ones that can be found in blood. If the drugs that are detectable in blood/urine are not all detectable in hair/urine how does that make hair/urine better?

The thing is you dont know either... maybe its more effective than blood since we are dealing with DNA. The best way is to get medical professionals the way NSAC did, to answer these questions.

In any case, all these test are just meant to appease both sides. DNA testing seems to be the best option given that it can be administer until the day of the fight (Floyd's demand), and would not be too intrusive (Pac's demand).

So basically with this test you will have one of the major impasse solved, which is a step closer in making the fight.

[Spoon23]

If it doesn't detect the same amount as blood/urine then it is not better, you have provided no proof that it does.

I'm finished here because you are obviously a little too slow to understand.

here's more:

Hair analysis of seven bodybuilders for anabolic steroids, ephedrine, and clenbuterol.

Dumestre-Toulet V, Cirimele V, Ludes B, Gromb S, Kintz P.

Laboratoire BIOffice, Artigues Pres Bordeaux, France. vdumestr@alienor.fr
Abstract

Several bodybuilders, all winners of international competitions, were arrested for trafficking of a number of doping agents including anabolic steroids, ephedrine, beta-adrenergics, human chorionic gonadotropin, antidepressants, and diuretics. In accordance with the recent French law against doping, the judge asked to test seven bodybuilders to identify doping practices. Hair and urine specimens were collected for analysis. After decontamination, a 100 mg hair strand was pulverized in a ball mill, hydrolyzed, extracted, and derivatized to be tested by GC/MS for anabolic steroids, beta-adrenergic compounds, ephedrine, and other doping agents. Urine was analyzed for anabolic steroids and metabolites, beta-adrenergic compounds, ephedrine, and human chorionic gonadotropin, in addition to a broad spectrum screening with GC/MS. The following compounds were detected in urine: ephedrine (29 and 36 ng/mL, n = 2), clenbuterol (0.2 to 0.3 ng/mL, n = 3), norandrosterone (4.7 to 100.7 ng/mL, n = 7), norethiocholanolone (0.9 to 161.8 ng/mL, n = 6), stanozolol (1 to 25.8 ng/mL, n = 4), methenolone (2.5 to 29.7 ng/mL, n = 4), testosterone (3 to 59.6 ng/mL, n = 7), epitestosterone (1 to 20.4 ng/mL, n = 7) and ratio testosterone/epitestosterone >6 for four subjects (18.5 to 59.6). The following drugs were detected in hair: ephedrine (0.67 and 10.70 ng/mg, n = 2), salbutamol (15 to 31 pg/mg, n = 3), clenbuterol (15 to 122 pg/mg, n = 6), nandrolone (1 to 7.5 pg/mg, n = 3), stanozolol (2 to 84 pg/mg, n = 4), methenolone (17 and 34 ng/ml, n = 2), testosterone enanthate (0.6 to 18.8 ng/mg, n = 5), and testosterone cypionate (3.3 to 4.8 ng/mg, n = 2).

These results document the doping practice and demonstrate repetitive exposure to anabolic compounds and confirm the value of hair analysis as a complement to urinalysis in the control of doping practice.

[IMDAZED]

Pacquiao lovers have gone from "only p*ssys ask for tougher testing" to trying to find tougher tests! These f*gs are out of control!

[Real King Kong]

throw that argument out of the window because the NSAC stated they WILL NOT take blood too closely to a fight.

and this is about making one fight happen. Not the fake crusade floyd is on and his dumb fans that seem to be wayyyyy too sold on "blood"

dude...once again i'm not a floyd fan...i'm a boxing fan. have you asked yourself why pacquiao has such a big problem with blood testing while other fighters don't? or why amateur boxers among other athletes use these same testing procedures with no adverse effects, yet pacquiao says it's no good? i care nothing of floyds crusade...i do however believe the testing procedures to date are not good enough. if they change good...if they don't...oh well. that won't be the only thing wrong with the sport...i'll still watch it.

[Spoon23]

If it doesn't detect the same amount as blood/urine then it is not better, you have provided no proof that it does.

I'm finished here because you are obviously a little too slow to understand.

here's more:

Detection of Anabolic Steroids in Head Hair

As we approach the new millennium we find the science of building muscle progressing faster than ever before. Long gone are the days of simple trial and error when it comes to building muscle. The modern bodybuilder demands more than just "hear say" if they are to adopt a new training routine or nutritional supplement. This column was created to keep today’s bodybuilder on the cutting edge of scientific research that might benefit them in their quest for body perfection.

Look for the "Bald Look" to be more than a fashion statement in the near future!

Title: Detection of anabolic steroids in head hair.

Researchers: Deng XS, Kurosu A, Pounder DJ

Department of Forensic Medicine, University of Dundee, Royal Infirmary, Scotland.

Source: J Forensic Sci 1999 Mar;44(2):343-6

Summary/Abstract: Scientists in Scotland have developed a gas chromatography/mass spectrometry method for detection and quantification of anabolic steroids in head hair. Following alkaline digestion and solid-phase extraction, the MO-TMS derivatives gave a specific fragmentation pattern with EI ionization. For stanozolol, the TMS-HFBA derivative showed several diagnostic ions. For androstanolone, mestanolone (methylandrostanolone), and oxymetholone two chromatographic peaks for cis and trans isomers of derivatives were seen. Recoveries were 35 to 45% for androstanolone, oxymetholone, chlorotestosterone-acetate, dehydromethyltestosterone, dehydrotestosterone, fluoxymesterone, mestanolone, methyltestosterone, and nandrolone; 52% for mesterolone, trenbolone; 65% for bolasterone; 24% for methenolone and 17% for stanozolol. Limits of detection were 0.002 to 0.05 ng/mg and of quantitation were 0.02 to 0.1 ng/mg. Seven white male steroid abusers provided head hair samples (10 to 63 mg) and urine. In the hair samples, methyltestosterone was detected in two (confirmed in urine); nandrolone in two (also confirmed in urine); dehydromethyltestosterone in four (but not found in urine); and clenbuterol in one (but not in urine). Oxymethalone was found in urine in one, but not in the hair. One abuser had high levels of testosterone: 0.15 ng/mg hair, and 1190 ng/mL urine. These researchers conclude that head hair analysis has considerable potential for the detection and monitoring of steroid abuse.

Discussion: When seeking to find out what chemicals are present in a sample (e.g., hair), you have to be able to effectively separate all the different substances it is made of. Usually this is done on the basis of differences in solubility, using fractional crystallization, or by differences in volatility, using distillation, of the different substances it contains.

Chromatography is the most widely used technique used in chemistry to separate substances. It can be used on liquid or gas solutions. It was originally used about 90 years ago to separate different color pigments in plants, hence the root "chroma" which is Greek for "color". Once a solution or gas is produced of the desired sample, the different chemicals in the sample will have different absorption characteristics in a solid or liquid phase medium. You may have heard or read the term "HPLC" when talking about testing the purity of supplements. HPLC refers to high-pressure liquid chromatography. With HPLC solutions are pumped through a column under very high pressure (3000/in2 or more). In this case researchers are using gas chromatography (GC). It is the same method used to test emissions on cars.

Mass spectrometry is another commonly used method of separating atomic sized particles in a gas based on weight. In mass spectrometry, a beam of gaseous ions is deflected in a magnetic field toward a collector plate. Lighter ions are deflected more than heavy ones. By comparing the accelerating voltages required to deflect the two ions to the same point on the collector plate, it is possible to determine the relative masses of the ions.

I’m sure most of you aren’t as interested in the technology as you are in the consequences of using it in drug testing. In this case researchers are using hair to determine what anabolics are or were taken.

Each hair on your body consists of a shaft and a root. The shaft consists of three principle parts. The inner medulla is composed of polyhedral cells containing granules of eleidin and air spaces. The middle cortex forms the major part of the shaft and consists of elongated cells that contain pigment granules in dark hair but mostly air in white hair. The cuticle of the hair, the outermost layer, consists of a single layer of thin, flat, scalelike cells that are most heavily keratinized.

It is these cells that contain metabolites of whatever anabolics you have been taking. The thing about hair is that it provides the investigator with a rather long history of the subjects drug use. Much longer than urine or blood. This is not the kind of test that can be fooled with masking agents. The only real way to beat this test at present is to remove all traces of hair from your body. Right now this study is only an indication of what can be done. It will be some time before this kind of testing would be adopted by major sporting organizations, if ever.

[Deevel916]

Pacquiao lovers have gone from "only p*ssys ask for tougher testing" to trying to find tougher tests! These f*gs are out of control!

Its been my stance from day 1 that Floyd is using the whole blood testing issue as a way to get into Pac's head. He knows Pac isnt fond of having blood drawn, and wants to use it as a psychological tool

Now if there's another accurate method that can catch ped's that's not intrusive, then what's the problem?

[Spoon23]

dude...once again i'm not a floyd fan...i'm a boxing fan. have you asked yourself why pacquiao has such a big problem with blood testing while other fighters don't? or why amateur boxers among other athletes use these same testing procedures with no adverse effects, yet pacquiao says it's no good? i care nothing of floyds crusade...i do however believe the testing procedures to date are not good enough. if they change good...if they don't...oh well. that won't be the only thing wrong with the sport...i'll still watch it.

enough already you hve been owned so many times in this thread lol go home and sleep it off hehe

[Rick Grimes]

here's more:

Detection of Anabolic Steroids in Head Hair

As we approach the new millennium we find the science of building muscle progressing faster than ever before. Long gone are the days of simple trial and error when it comes to building muscle. The modern bodybuilder demands more than just "hear say" if they are to adopt a new training routine or nutritional supplement. This column was created to keep today’s bodybuilder on the cutting edge of scientific research that might benefit them in their quest for body perfection.

Look for the "Bald Look" to be more than a fashion statement in the near future!

Title: Detection of anabolic steroids in head hair.

Researchers: Deng XS, Kurosu A, Pounder DJ

Department of Forensic Medicine, University of Dundee, Royal Infirmary, Scotland.

Source: J Forensic Sci 1999 Mar;44(2):343-6

Summary/Abstract: Scientists in Scotland have developed a gas chromatography/mass spectrometry method for detection and quantification of anabolic steroids in head hair. Following alkaline digestion and solid-phase extraction, the MO-TMS derivatives gave a specific fragmentation pattern with EI ionization. For stanozolol, the TMS-HFBA derivative showed several diagnostic ions. For androstanolone, mestanolone (methylandrostanolone), and oxymetholone two chromatographic peaks for cis and trans isomers of derivatives were seen. Recoveries were 35 to 45% for androstanolone, oxymetholone, chlorotestosterone-acetate, dehydromethyltestosterone, dehydrotestosterone, fluoxymesterone, mestanolone, methyltestosterone, and nandrolone; 52% for mesterolone, trenbolone; 65% for bolasterone; 24% for methenolone and 17% for stanozolol. Limits of detection were 0.002 to 0.05 ng/mg and of quantitation were 0.02 to 0.1 ng/mg. Seven white male steroid abusers provided head hair samples (10 to 63 mg) and urine. In the hair samples, methyltestosterone was detected in two (confirmed in urine); nandrolone in two (also confirmed in urine); dehydromethyltestosterone in four (but not found in urine); and clenbuterol in one (but not in urine). Oxymethalone was found in urine in one, but not in the hair. One abuser had high levels of testosterone: 0.15 ng/mg hair, and 1190 ng/mL urine. These researchers conclude that head hair analysis has considerable potential for the detection and monitoring of steroid abuse.

Discussion: When seeking to find out what chemicals are present in a sample (e.g., hair), you have to be able to effectively separate all the different substances it is made of. Usually this is done on the basis of differences in solubility, using fractional crystallization, or by differences in volatility, using distillation, of the different substances it contains.

Chromatography is the most widely used technique used in chemistry to separate substances. It can be used on liquid or gas solutions. It was originally used about 90 years ago to separate different color pigments in plants, hence the root "chroma" which is Greek for "color". Once a solution or gas is produced of the desired sample, the different chemicals in the sample will have different absorption characteristics in a solid or liquid phase medium. You may have heard or read the term "HPLC" when talking about testing the purity of supplements. HPLC refers to high-pressure liquid chromatography. With HPLC solutions are pumped through a column under very high pressure (3000/in2 or more). In this case researchers are using gas chromatography (GC). It is the same method used to test emissions on cars.

Mass spectrometry is another commonly used method of separating atomic sized particles in a gas based on weight. In mass spectrometry, a beam of gaseous ions is deflected in a magnetic field toward a collector plate. Lighter ions are deflected more than heavy ones. By comparing the accelerating voltages required to deflect the two ions to the same point on the collector plate, it is possible to determine the relative masses of the ions.

I’m sure most of you aren’t as interested in the technology as you are in the consequences of using it in drug testing. In this case researchers are using hair to determine what anabolics are or were taken.

Each hair on your body consists of a shaft and a root. The shaft consists of three principle parts. The inner medulla is composed of polyhedral cells containing granules of eleidin and air spaces. The middle cortex forms the major part of the shaft and consists of elongated cells that contain pigment granules in dark hair but mostly air in white hair. The cuticle of the hair, the outermost layer, consists of a single layer of thin, flat, scalelike cells that are most heavily keratinized.

It is these cells that contain metabolites of whatever anabolics you have been taking. The thing about hair is that it provides the investigator with a rather long history of the subjects drug use. Much longer than urine or blood. This is not the kind of test that can be fooled with masking agents. The only real way to beat this test at present is to remove all traces of hair from your body. Right now this study is only an indication of what can be done. It will be some time before this kind of testing would be adopted by major sporting organizations, if ever.

Cong**** you have shown what we have known for the last 9 pages that it can detect some PED's, still nowhere does it say that it can detect the same amount or more than blood. Keep trying...

[Spoon23]

Its been my stance from day 1 that Floyd is using the whole blood testing issue as a way to get into Pac's head. He knows Pac isnt fond of having blood drawn, and wants to use it as a psychological tool

Now if there's another accurate method that can catch ped's that's not intrusive, then what's the problem?

Bro it's pointless talking to *****s, especially that guy you just replied to that's the sickest badass gay ***** ever lol

That guy doesn't know common sense, logic is not part of his vocabulary. Do yourself a favor, don't go to his level my man. It ain't worth talking to a dumbass blockhead hehe!

[mikemurni]

dude...once again i'm not a floyd fan...i'm a boxing fan. have you asked yourself why pacquiao has such a big problem with blood testing while other fighters don't? or why amateur boxers among other athletes use these same testing procedures with no adverse effects, yet pacquiao says it's no good? i care nothing of floyds crusade...i do however believe the testing procedures to date are not good enough. if they change good...if they don't...oh well. that won't be the only thing wrong with the sport...i'll still watch it.

Actually Pac is not the only professional athlete that have refused blood test.

http://sports.espn.go.com/nfl/news/story?id=4940922