The U.S. stranglehold on the women's middleweight division is no longer. 

Naomi Graham fell short in her Round of 16 matchup with Zenfira Magomedalieva (ROC), losing on four of the five scorecards Wednesday evening at Kokugikan Arena in Tokyo. 

Scores were 30-27, 30-27, 29-28 and 29-28 for Magomedalieva and 29-28.

The 33-year-old Graham entered Tokyo as the number-three seed but did not pull a favorable draw in the opening round of middleweight competition. Magomedalieva began her amateur career as a heavyweight after having previously bulked up while as a shot putter, before working her way down the scale.

Magomedalieva worked her right hand throughout the fight, with Graham unable to contend other than through tying up the ROC (Russian Olympic Committee) athlete. That tactic was forced to serve as a go-to more than was preferred by the current US Army Staff Sergeant, the first-ever female to represent USA in the Olympics while serving active duty. 

The night never got much better for Graham, though she managed to win rounds two and three on the scorecard of Mongolia judge Tsogtgerel Tserenkhand and on three of the five cards overall for round three. 

Unfortunately, it was too little too late for the North Carolina native, who becomes just the second U.S. boxer to be eliminated from competition. Yarisel Ramirez lost a split decision in the opening round of featherweight competition on Saturday, with the U.S. squad going on to win their next eight bouts. 

The eight-fight win streak put their record at 8-1 prior to Graham's bout, matching the same start enjoyed by the 2016 U.S. team who went on to win three medals. One came at middleweight, where Claressa Shields—self-referenced as the GWOAT (Greatest Woman Of All Time) of boxing—captured her second straight Gold medal. 

The division's top prize will now land in the hands of another nation. 

 USA is now 8-2 in Tokyo—6-0 on the men's side and 2-2 among the five-women squad. 

Jake Donovan is a senior writer for Twitter: @JakeNDaBox