Originally posted by _Rexy_
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Understandably it won’t mean much for those with no laboratory experience but for those who do, it can be elucidating. For example, DOA metabolites are tested via rudimentary bioanalyzers for most commercial applications. They are semi quantitative test, meaning after measuring absorbance there’s a normalize signal that’s quantified as a function of concentration, but not in the same way a real time PCR would give a reading. The quantity is then referenced against a cut off value that determines whether it’s positive/negative hence the qualitative aspect.
The reason I bring this up is that I’ve got a garbage bioanalyzer in the lab that I manage. It’s competent enough to run and pass the QC but there’s a moderate amount variability in precision and accuracy. There’s been times that samples have come up positive that shouldn’t have based off of patient history, but upon repeat and confirmation from more sensitive assays (mass spec) they returned negative.
Urine samples should be tested in replicate on both an Elisa and mass spec assay. As someone who’s worked in both clinical and industry laboratory science, having these protocols to maintain test integrity are universally embraced, but there’s always failings in many places I’ve worked in. Let alone in a landscape where corruption is rampant (ie boxing).
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