Glenn Rushton, the head trainer of Jeff Horn, has made it clear that there won't be a trilogy fight with Michael Zerafa in the aftermath of their rematch.

Rushton is angered over what he perceives as a lack of respect from the Zerafa camp, as they head in to Wednesday night's middleweight bout in Brisbane.

Rushton warned the Zerafa team that Horn is very much improved since getting stopped by Zerafa in nine rounds back in August.

After Horn tasted defeat, he exercised his contractual clause for an immediate rematch.

"After the amount of disrespect, it will be the only time probably that I haven't gone over to another fighter's dressing room, because I'd just rather walk away, from that whole team and we'll move on," Rushton said.

"There will be no third fight, there will be no more fights with Michael Zerafa, regardless of the outcome, no interest."

Horn backed his trainer's position on a trilogy.

"They are very disrespectful, the way they talk about Glenn and he's a rubbish trainer and the way they talk about me that I'm a rubbish fighter," Horn said.

"It's unheard of what they are saying, it's stupid. It does give me extra motivation, when they are saying these type of things, to want to win this fight."

But Zerafa hit back, stating that the lack of respect is mutual. Horn, who captured a world title at welterweight, jumped all the way up to middleweight for the bouts with Zerafa - which some believe was a weight limit that is too high for him.

"I've trained super hard, my strength, my power, my mental ability has gone through the roof; I'm just on another level," Zerafa said.